Akoya Biosciences PhenoCycler Fusion (formerly CODEX) User Guide
Andrew Houston
Published: 2024-01-12 DOI: 10.17504/protocols.io.x54v9p4j1g3e/v1
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Abstract
Link to PhenoCycler User Guide to allow for multi-plex spatial proteomic detection on fresh frozen and FFPE tissue sections
Steps
1.
Staining/slide prep protocol can be found here:
PhenoCycler-Fusion User Guide_2.1.0.pdf
FFPE tissue staining pgs. 45-56
Reporter plate preparation pgs. 70-75
2.
Imaging protocol can be found here:
PhenoImager Fusion User Guide_V1_1 Flow Cell_ 2.1.0.pdf
Flow cell assembly pgs. 29-39
Instrument set-up for PhenoCycler scan pgs. 57-71
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