Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq

J. Keith Joung, Martin J. Aryee, Nikolay L. Malinin, GaHyun Lee, Cicera R. Lazzarotto, Yichao Li, Zongli Zheng, Nhu T. Nguyen, Matthew Liebers, Ved V. Topkar, A. John Iafrate, Long P. Le, Shengdar Q. Tsai

Published: 2021-11-11 DOI: 10.1038/s41596-021-00626-x

off-target sites

high-throughput sequencing

GUIDE-seq

genome-editing

nuclease activity

AI 解读

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Heterogeneity（异质性）

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Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of effi
Introduction
Materials
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Anticipated results
References
ACKNOWLEDGMENTS

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Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq

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