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    Habituation and dishabituation (olfaction test)

    Ariadna Laguna, Miquel Vila, Núria Peñuelas, Marina Lorente Picón

    Published: 2023-12-01 DOI: 10.17504/protocols.io.e6nvwdq9dlmk/v1
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    Abstract

    Habituation and dishabituation (olfaction test) for mice

    Steps

    1.

    Individualize each mouse in a different cage with new bedding.

    2.

    Present one cotton swab to the animal for object habituation for 10 min.

    3.

    Present a second swab impregnated with water for 3 min.

    4.

    Measure the number of times the animal goes towards the second cotton swab and the time the animal spends sniffing it.

    5.

    Present a third swab impregnated with lemon essence (Essenciales) for 3 min.

    6.

    Measure the number of times the animal goes towards the third cotton swab and the time the animal spends sniffing it.

    7.

    Calculate the discrimination index (DI) according to the formula: (Time exploring lemon essence – Time exploring water) / (Time exploring lemon essence + Time exploring water).

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