Bacterial genome engineering using CRISPR-associated transposases

Diego Rivera Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris H. Wang, Samuel H. Sternberg

Published: 2024-01-12 DOI: 10.1038/s41596-023-00927-3

genome engineering

CRISPR-associated transposases

bacterial genomes

DNA insertions

multiplexed edits

AI 解读

Supplementary information

Reporting Summary

Supplementary Tables 1–3

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Supplementary information

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Bacterial genome engineering using CRISPR-associated transposases

Supplementary information

Reporting Summary

Supplementary Tables 1–3

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