High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ

Jianshi Jin, Reiko Yamamoto, Katsuyuki Shiroguchi

Published: 2023-11-27 DOI: 10.1038/s41596-023-00906-8

Barcoding

Microbiota

16S rRNA Sequencing

Single-Base Accuracy

Bacterial Quantification

AI 解读

Supplementary information

Supplementary Information

Supplementary Methods 1 and 2.

Reporting Summary

Supplementary Table 1

Information of oligomers and barcodes.

Supplementary Table 2

16S rRNA sequences of bacteria in mock community.

Supplementary Table 3

Quantification of bacteria in mock community.

Supplementary Table 4

16S rRNA sequences of bacteria in cecal samples.

Supplementary Table 5

Quantification of bacteria in cecal samples.

Supplementary Data 1

Example of ‘sample sheet’ for a MiSeq run.

Supplementary Data 2

16S rRNA sequences of cecal samples in fasta format with predicted taxonomies.

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Supplementary information

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