Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors

Jana Van Dycke, Arno Cuvry, Jan Knickmann, Annelii Ny, Sebastian Rakers, Stefan Taube, Peter de Witte, Johan Neyts, Joana Rocha-Pereira

Published: 2021-04-08 DOI: 10.1038/s41596-021-00499-0

zebrafish larvae

human norovirus

viral replication

small-molecule inhibitors

antiviral efficacy

AI 解读

Supplementary information

Reporting Summary

Supplementary Video 1

Clipping of the microinjection needle

Supplementary Video 2

Correct size bolus

Supplementary Video 3

Microinjection in the yolk of 3 dpf zebrafish larvae

Supplementary Video 4

Dechorionation of 2 dpf zebrafish larvae

Supplementary Video 5

Microinjection in the pericardial cavity of 3 dpf zebrafish larvae

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Supplementary information

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