Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

Anais Sahabian, Julia Dahlmann, Ulrich Martin, Ruth Olmer

Published: 2021-02-11 DOI: 10.1038/s41596-020-00470-5

human pluripotent stem cells

definitive endoderm

cryopreservation

scalable culture

endodermal lineages

AI 解读

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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The endodermal germ layer gives rise to respiratory epithelium, hepatocytes, pancreatic cells and intestinal lineages, among other cell types. These lineages can be differentiated from human pluripotent stem cells (hPSCs) via a common definitive endoderm
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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ACKNOWLEDGMENTS

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Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions

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